Quantification of Amylase Enzyme from the Calcite Residing Actinobacteria Isolated from Limestone Quarries of Deccan Traps

Syed Raziuddin Quadri1,2,3, Jamal Al Ruwaili1,Shaik Mohammed Akbar5, Wen-Jun Li3,4 and Dayanand Agsar2 Sixty three actinobacteria were isolated from Limestone quarries of Deccan Traps from South India. The isolates were identified by morphological, biochemical and physiological studies. Among these, six isolates were selected on the basis of their amylolytic activity using submerged cultivation. The potential isolates exhibiting amylolytic activity were DRQ10, DRQ20, DRQ86, DRQ33, DRQ41, and DRQ63 as confirmed by formation of clear zones of starch hydrolysis around the colonies, with maximum activities 2500, 1100, 1200, 1150, 1250, 1400, and 1600 Units, respectively, as determined by DNS (3...

Sinomonas mesophila sp. nov., isolated from ancient fort soil

Deene Manik Prabhu1,2,7, Syed Raziuddin Quadri3,7, Juan Cheng4, Lan Liu4, Wei Chen5, Ying Yang5,Wael N Hozzein6, Kattimani Lingappa2 and Wen-Jun Li1,4 A moderate growing strain designated as MPKL 26 T was isolated from a soil sample of Bidar Fort, Karnataka, India. The strain MPKL 26T was Gram positive, bent rod in shape. The optimum pH and temperature for growth was 7.0 and 30 °C, respectively. The 16S ribosomal RNA gene sequence analysis revealed that strain MPKL 26 T was closely related to S. atrocyanea DSM 20127T (98.09%), S. flava CW 108T (98.04%), S. soli CW 59T (97.99%) and S. notoginsengisoli SYP-B575T (97.0%) and showed DNA–DNA hybridization relatedness (46.05 ±1.2, 33.56 ±2.55, 32...

Proteolytic Activity from an Alkali-Thermotolerant Streptomyces gulbargensis sp. nov.

S. G. Dastager Æ Agasar Dayanand Æ Wen-Jun Li Æ Chang-Jin Kim Æ Jae-Chan Lee Æ Dong-Jin Park Æ Xin-Peng Tian Æ Q. S. Raziuddin Received: 9 April 2007 / Accepted: 13 January 2008  Springer Science+Business Media, LLC 2008 Abstract Multiple proteases were produced and partially purified from an alkali-thermotolerant novel species of Streptomyces (i.e., Streptomyces gulbargensis DAS 131) after 48 h of growth at 45C. The enzyme preparation exhibited activity over a broad range of pH (4–12) and temperature (27–55C). Optimum activity was observed at a pH of 9.0 and a temperature of 45C. Starch and protease peptone was found to be a good source of carbon and nitrogen to enhance the enzyme...

ANTIMICROBIAL ATTRIBUTES OF RARE ACTINOBACTERIA DETECTED FROM LIMESTONE QUARRIES

SYED RAZIUDDIN QUADRI AND DAYANAND AGSAR Rare actinobacterial isolates belonging to the genera Micromonospora, Nonomuraea, Kribbella, Lechevalieria, Saccharotherix and commonly occurring Streptomyces were isolated from Limestone quarry - a unique and geographical area and harsh habitat. pH – 9.0, temperature – 45°C and sodium chloride concentration – 3% were found to be optimum for the maximum growth and physiological ability of the test isolates indicating them as thermo-alkaliphilic in nature. It was found that, the test isolate DRQ 10 belonging to the genus Streptomyces and DRQ 72 belonging to the genus Micromonospora were very effective showing maximum zone of inhibition against the...

Detection of melanin producing thermo-alkaliphilic Streptomyces from limestone quarries of the Deccan traps

Syed Raziuddin Quadri and Dayanand Agsar Streptomyces is a prominent genus under actinomycetes and well known for the synthesis of variety of bioactive molecules. In the present study, eight Streptomyces species were isolated and characterized from the soil samples of limestone quarries of the Deccan traps, India. The identified organisms being thermo-alkaliphilic, alkaline pH (9.0), higher temperature (45oC) and 3.0 % concentration of sodium chloride were proved to be important physiological attributes for the more synthesis of melanin. DRQ 11, 12 and 26 have been proved as efficient isolates for the increased synthesis of melanin - 2.76 µg/ml, 2.66 µg/ml and 2.85 µg/ml in Shinobu's...

Pontibacter niistensis sp. nov., isolated from forest soil

Syed G. Dastager, 1 Q. S. Raziuddin,2 C. K. Deepa,1 Wen-Jun Li3 and Ashok Pandey1 A Gram-negative, rod-shaped bacterial strain, NII-0905 T, that was motile by gliding was isolated from soil of a dense forest collected from the Western Ghats of India and its taxonomic position was established. Strain NII-0905T contained MK-7 as the major menaquinone and anteiso-C17 : 0, anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major cellular fatty acids. The DNA G+C content of strain NII-0905 T was 51.47 mol%. 16S rRNA gene sequence-based phylogenetic analysis confirmed the placement of strain NII-0905T in the genus Pontibacter and strain NII-0905T exhibited 93.9–96.3 % 16S rRNA sequence similarity...